axio observer z 1 inverted epifluorescence microscope Search Results


axio observer z1 inverted epifluorescence microscope  (Carl Zeiss)
99
Carl Zeiss axio observer z1 inverted epifluorescence microscope
Axio Observer Z1 Inverted Epifluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescence inverted microscope  (Carl Zeiss)
98
Carl Zeiss epifluorescence inverted microscope
Epifluorescence Inverted Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescence microscope zeiss axio observer z1  (Carl Zeiss)
96
Carl Zeiss epifluorescence microscope zeiss axio observer z1
Epifluorescence Microscope Zeiss Axio Observer Z1, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axio observer z1 7 epifluorescence microscope  (Carl Zeiss)
98
Carl Zeiss axio observer z1 7 epifluorescence microscope
Axio Observer Z1 7 Epifluorescence Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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wide field epifluorescence zeiss axio observer z1 microscope  (Carl Zeiss)
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Carl Zeiss wide field epifluorescence zeiss axio observer z1 microscope
Wide Field Epifluorescence Zeiss Axio Observer Z1 Microscope, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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orca-er camera  (Hamamatsu)
90
Hamamatsu orca-er camera
Orca Er Camera, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axio observer z1 7  (Carl Zeiss)
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Carl Zeiss axio observer z1 7
Axio Observer Z1 7, supplied by Carl Zeiss, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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epifluorescence  (Nikon)
99
Nikon epifluorescence
Epifluorescence, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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axio observer z1 epifluorescence microscope  (Hamamatsu)
90
Hamamatsu axio observer z1 epifluorescence microscope
Axio Observer Z1 Epifluorescence Microscope, supplied by Hamamatsu, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/axio+observer+z+1+inverted+epifluorescence+microscope/axio+observer+z1+epifluorescence+microscope/bio_rxiv__2022__09__29__510093-155-19-29
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water-cooled cmos camera (orca flash 4  (Hamamatsu)
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o 2 controller  (PeCon GmbH)
90
PeCon GmbH o 2 controller
Sub-nuclear localization of HIF-1α and HIF-2α. ( a ) HeLa cells ectopically expressing HIF-1α and HIF-2α EGFP fusions compared with endogenous HIF-1α and HIF-2α labelled using immunostaining. Images of HIF-1α were taken following DMOG treatment (6 h; 0.5 mM). Scale bar, 5 μm. ( b ) HeLa cells transiently transfected with plasmids encoding (i) clover-HIF-2α (green, pseudocolour), (ii) dsRED-HIF-2α (red, pseudocolour), (iii) HIF-2α-venus (yellow pseudocolour) and (iv) Halotag-HIF-2α (green, pseudocolour). The cells expressing Halotag-HIF-2α were labelled with the fluorescent Oregon Green Halotag ligand (HL-OregonGreen; Promega, WI, USA) to visualize the fusion protein. ( c ) Confocal images of C2C12 (mouse myoblast; top) and HEK293T (Human embryonic kidney cells; bottom) cells ectopically expressing EGFP-HIF-2α. Scale bar, 5 μm. ( d ) HeLa cells transiently transfected with EGFP-HIF-2α were imaged with a CCD camera. One thousand frames were acquired per cell in normoxia, hypoxia (1% v/v <t>O</t> <t>2</t> , 16 h) or following treatment with DMOG (0.5 mM, 6 h). The average (±s.d.) number of speckles per nucleus in each condition was 64 ± 49 ( n = 25), 44 ± 24 ( n = 24) and 96 ± 33 ( n = 22), respectively. Mean of the sample data represented by the red dashed line. ( e ) Using the images from ( d ) the average speckle area per nucleus over the 1000 frames. The mean values (±s.d.) for each condition were 0.24 ± 0.09 µm ( n = 25), 0.21 ± 0.07 µm ( n = 24) and 0.27 ± 0.09 µm ( n = 22), respectively. The mean values for hypoxia and DMOG were compared with the normoxic values (independent t -test, significance value set at 5%). Mean of the sample data represented by the red dashed line.
O 2 Controller, supplied by PeCon GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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eclipse ts100 nikon epifluorescence inverted microscope  (Nikon)
96
Nikon eclipse ts100 nikon epifluorescence inverted microscope
Sub-nuclear localization of HIF-1α and HIF-2α. ( a ) HeLa cells ectopically expressing HIF-1α and HIF-2α EGFP fusions compared with endogenous HIF-1α and HIF-2α labelled using immunostaining. Images of HIF-1α were taken following DMOG treatment (6 h; 0.5 mM). Scale bar, 5 μm. ( b ) HeLa cells transiently transfected with plasmids encoding (i) clover-HIF-2α (green, pseudocolour), (ii) dsRED-HIF-2α (red, pseudocolour), (iii) HIF-2α-venus (yellow pseudocolour) and (iv) Halotag-HIF-2α (green, pseudocolour). The cells expressing Halotag-HIF-2α were labelled with the fluorescent Oregon Green Halotag ligand (HL-OregonGreen; Promega, WI, USA) to visualize the fusion protein. ( c ) Confocal images of C2C12 (mouse myoblast; top) and HEK293T (Human embryonic kidney cells; bottom) cells ectopically expressing EGFP-HIF-2α. Scale bar, 5 μm. ( d ) HeLa cells transiently transfected with EGFP-HIF-2α were imaged with a CCD camera. One thousand frames were acquired per cell in normoxia, hypoxia (1% v/v <t>O</t> <t>2</t> , 16 h) or following treatment with DMOG (0.5 mM, 6 h). The average (±s.d.) number of speckles per nucleus in each condition was 64 ± 49 ( n = 25), 44 ± 24 ( n = 24) and 96 ± 33 ( n = 22), respectively. Mean of the sample data represented by the red dashed line. ( e ) Using the images from ( d ) the average speckle area per nucleus over the 1000 frames. The mean values (±s.d.) for each condition were 0.24 ± 0.09 µm ( n = 25), 0.21 ± 0.07 µm ( n = 24) and 0.27 ± 0.09 µm ( n = 22), respectively. The mean values for hypoxia and DMOG were compared with the normoxic values (independent t -test, significance value set at 5%). Mean of the sample data represented by the red dashed line.
Eclipse Ts100 Nikon Epifluorescence Inverted Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 96 stars, based on 1 article reviews
eclipse ts100 nikon epifluorescence inverted microscope - by Bioz Stars, 2026-09
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Image Search Results


Sub-nuclear localization of HIF-1α and HIF-2α. ( a ) HeLa cells ectopically expressing HIF-1α and HIF-2α EGFP fusions compared with endogenous HIF-1α and HIF-2α labelled using immunostaining. Images of HIF-1α were taken following DMOG treatment (6 h; 0.5 mM). Scale bar, 5 μm. ( b ) HeLa cells transiently transfected with plasmids encoding (i) clover-HIF-2α (green, pseudocolour), (ii) dsRED-HIF-2α (red, pseudocolour), (iii) HIF-2α-venus (yellow pseudocolour) and (iv) Halotag-HIF-2α (green, pseudocolour). The cells expressing Halotag-HIF-2α were labelled with the fluorescent Oregon Green Halotag ligand (HL-OregonGreen; Promega, WI, USA) to visualize the fusion protein. ( c ) Confocal images of C2C12 (mouse myoblast; top) and HEK293T (Human embryonic kidney cells; bottom) cells ectopically expressing EGFP-HIF-2α. Scale bar, 5 μm. ( d ) HeLa cells transiently transfected with EGFP-HIF-2α were imaged with a CCD camera. One thousand frames were acquired per cell in normoxia, hypoxia (1% v/v O 2 , 16 h) or following treatment with DMOG (0.5 mM, 6 h). The average (±s.d.) number of speckles per nucleus in each condition was 64 ± 49 ( n = 25), 44 ± 24 ( n = 24) and 96 ± 33 ( n = 22), respectively. Mean of the sample data represented by the red dashed line. ( e ) Using the images from ( d ) the average speckle area per nucleus over the 1000 frames. The mean values (±s.d.) for each condition were 0.24 ± 0.09 µm ( n = 25), 0.21 ± 0.07 µm ( n = 24) and 0.27 ± 0.09 µm ( n = 22), respectively. The mean values for hypoxia and DMOG were compared with the normoxic values (independent t -test, significance value set at 5%). Mean of the sample data represented by the red dashed line.

Journal: Open Biology

Article Title: Differential sub-nuclear distribution of hypoxia-inducible factors (HIF)-1 and -2 alpha impacts on their stability and mobility

doi: 10.1098/rsob.160195

Figure Lengend Snippet: Sub-nuclear localization of HIF-1α and HIF-2α. ( a ) HeLa cells ectopically expressing HIF-1α and HIF-2α EGFP fusions compared with endogenous HIF-1α and HIF-2α labelled using immunostaining. Images of HIF-1α were taken following DMOG treatment (6 h; 0.5 mM). Scale bar, 5 μm. ( b ) HeLa cells transiently transfected with plasmids encoding (i) clover-HIF-2α (green, pseudocolour), (ii) dsRED-HIF-2α (red, pseudocolour), (iii) HIF-2α-venus (yellow pseudocolour) and (iv) Halotag-HIF-2α (green, pseudocolour). The cells expressing Halotag-HIF-2α were labelled with the fluorescent Oregon Green Halotag ligand (HL-OregonGreen; Promega, WI, USA) to visualize the fusion protein. ( c ) Confocal images of C2C12 (mouse myoblast; top) and HEK293T (Human embryonic kidney cells; bottom) cells ectopically expressing EGFP-HIF-2α. Scale bar, 5 μm. ( d ) HeLa cells transiently transfected with EGFP-HIF-2α were imaged with a CCD camera. One thousand frames were acquired per cell in normoxia, hypoxia (1% v/v O 2 , 16 h) or following treatment with DMOG (0.5 mM, 6 h). The average (±s.d.) number of speckles per nucleus in each condition was 64 ± 49 ( n = 25), 44 ± 24 ( n = 24) and 96 ± 33 ( n = 22), respectively. Mean of the sample data represented by the red dashed line. ( e ) Using the images from ( d ) the average speckle area per nucleus over the 1000 frames. The mean values (±s.d.) for each condition were 0.24 ± 0.09 µm ( n = 25), 0.21 ± 0.07 µm ( n = 24) and 0.27 ± 0.09 µm ( n = 22), respectively. The mean values for hypoxia and DMOG were compared with the normoxic values (independent t -test, significance value set at 5%). Mean of the sample data represented by the red dashed line.

Article Snippet: Cells expressing EGFP-HIF-2α were mounted on the stage of an Axio Observer Z.1 Epifluorescent microscope (Zeiss, Oberkochen, Germany) fitted with an incubation system and O 2 controller (PeCon GmBh, Erbach, Germany).

Techniques: Expressing, Immunostaining, Transfection